Replica-Plating Virulence Assay for Agrobacterium tumefaciens
نویسندگان
چکیده
منابع مشابه
Virulence of Agrobacterium tumefaciens Strain A281 on Legumes.
This study addresses the basis of host range on legumes of Agrobacterium tumefaciens strain A281, an l,l-succinamopine strain. We tested virulence of T-DNA and vir region constructs from this tumor-inducing (Ti) plasmid with complementary Ti plasmid regions from heterologous nopaline and octopine strains.
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Agrobacterium tumefaciens biovar 3 causes both crown gall and root decay of grapes. All biovar 3 strains, regardless of their tumorigenicity, produce in culture a single polygalacturonase with a pI around 4.5. A. tumefaciens biovar 3 strain CG49 was mutagenized with Tn5 by using pSUP2021 as a suicide vector. A mutant strain, CG50, lacking polygalacturonase activity was isolated. The mutation wa...
متن کاملVirulence of Agrobacterium tumefaciens requires phosphatidylcholine in the bacterial membrane.
Phosphatidylcholine (PC, lecithin) has long been considered a solely eukaryotic membrane lipid. Only a minority of all bacteria is able to synthesize PC. The plant-transforming bacterium Agrobacterium tumefaciens encodes two potential PC forming enzymes, a phospholipid N-methyltransferase (PmtA) and a PC synthase (Pcs). We show that PC biosynthesis and tumour formation on Kalanchoë plants was i...
متن کاملQuantitative aspects of transformation of virulence in Agrobacterium tumefaciens.
In the last decade, a number of bacterial species were shown to possess the ability to transmit morphological and biochemical characters to deficient strains. Tumor-inducing ability or virulence in crown-gall bacteria is such a biochemical character, and the acquisition of virulence is here taken to be the acquisition by avirulent strains of bacteria of the ability to synthesize a tumor-inducin...
متن کاملCorn metabolites affect growth and virulence of Agrobacterium tumefaciens.
Homogenates of corn seedlings inhibit both growth of Agrobacterium tumefaciens and induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS). The heat-labile inhibitor has been identified as 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), present in 2-week-old seedlings (B73) at a concentration of 1.5 mM or greater. A concentration of 0.3 mM DIMBOA is sufficient to ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1977
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.16.3.1027-1028.1977